基于诱导期的新型动力学方法测定痕量硫氰酸盐
Determination of trace amounts of thiocyanate by a new kinetic procedure based on an induction period.
作者信息
Chamjangali M Arab, Bagherian G, Salek-Gilani N
机构信息
College of Chemistry, Shahrood University of Technology, P.O. Box 36155, Shahrood 316, Iran.
出版信息
Spectrochim Acta A Mol Biomol Spectrosc. 2007 Aug;67(5):1252-6. doi: 10.1016/j.saa.2006.10.015. Epub 2006 Oct 14.
A new, simple and sensitive kinetic spectrophotometric method with no need for removing of interfering substances is proposed for the determination of thiocyanate ion in biological and water samples. The procedure is based on the inhibiting effect of thiocyanate on the sodium periodate-potassium bromide-meta cresol purple (MCP) system in acidic media. The induction period of the reaction is proportional to the SCN- concentration. The decolorization of meta cresol purple by the reaction products was used to monitor the reaction spectrophotometrically at 525 nm. Under optimum conditions, thiocyanate can be determined in the range of 0.02-0.8 microg ml(-1) with a 3sigma detection limit of 5 ng ml(-1). The relative standard deviations for 10 replicate determinations of 0.060, 0.10 and 0.50 microg ml(-1) thiocyanate are 3.7, 2.4 and 1.0%, respectively. This method has been successfully used to the determination of thiocyanate content in smokers and non-smokers saliva and spiked water sample.
提出了一种无需去除干扰物质的新型、简单且灵敏的动力学分光光度法，用于测定生物和水样中的硫氰酸根离子。该方法基于硫氰酸根在酸性介质中对高碘酸钠 - 溴化钾 - 间甲酚紫（MCP）体系的抑制作用。反应的诱导期与SCN⁻浓度成正比。利用反应产物使间甲酚紫褪色，在525 nm处用分光光度法监测反应。在最佳条件下，硫氰酸根的测定范围为0.02 - 0.8 μg ml⁻¹，3σ检测限为5 ng ml⁻¹。对0.060、0.10和0.50 μg ml⁻¹硫氰酸根进行10次重复测定的相对标准偏差分别为3.7%、2.4%和1.0%。该方法已成功用于测定吸烟者和非吸烟者唾液以及加标水样中的硫氰酸根含量。
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