Finnie J W, Van den Heuvel C, Gebski V, Manavis J, Summersides G E, Blumbergs P C
Veterinary Services Division and Neuropathology Laboratory, Institute of Medical and Veterinary Science, Adelaide, South Australia 5000, Australia.
J Comp Pathol. 2001 Feb-Apr;124(2-3):159-64. doi: 10.1053/jcpa.2000.0446.
The heads of anaesthetized lambs aged 4-5 weeks were subjected to impact (temporal, frontal or occipital) of constant strength with a humane stunner. Two hours later, the brains were perfusion-fixed with 4% paraformaldehyde and serial whole coronal slices processed by routine methods. Sections were stained with haematoxylin and eosin or labelled with a monoclonal antibody to amyloid precursor protein, a sensitive marker of axonal injury and neuronal reaction. Microscopical evaluation of axonal, neuronal and vascular damage was performed with a quantitative grid system. Frontal impact produced the greatest damage, followed by occipital then temporal impact. An unusual lesion found in the majority of lambs subjected to impact was multifocal necrosis of the cerebellar granular layer. The findings should assist clinicians in evaluating the probable outcome of traumatic head injury in domestic animals.
对4 - 5周龄的麻醉羔羊头部，使用人道击晕器施以恒定强度的撞击（颞部、额部或枕部）。两小时后，用4%多聚甲醛对大脑进行灌注固定，并按常规方法处理连续的全冠状切片。切片用苏木精和伊红染色，或用抗淀粉样前体蛋白单克隆抗体标记，淀粉样前体蛋白是轴突损伤和神经元反应的敏感标志物。使用定量网格系统对轴突、神经元和血管损伤进行显微镜评估。额部撞击造成的损伤最大，其次是枕部撞击，然后是颞部撞击。在大多数受撞击的羔羊中发现的一种不寻常病变是小脑颗粒层的多灶性坏死。这些发现应有助于临床医生评估家畜头部创伤的可能预后。
