Sharkey C M, North C L, Kuhn R J, Sanders D A
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907-1392, USA.
J Virol. 2001 Mar;75(6):2653-9. doi: 10.1128/JVI.75.6.2653-2659.2001.
Pseudotyped retroviruses have important applications as vectors for gene transfer and gene therapy and as tools for the study of viral glycoprotein function. Recombinant Moloney murine leukemia virus (Mo-MuLV)-based retrovirus particles efficiently incorporate the glycoproteins of the alphavirus Ross River virus (RRV) and utilize them for entry into cells. Stable cell lines that produce the RRV glycoprotein-pseudotyped retroviruses for prolonged periods of time have been constructed. The pseudotyped viruses have a broadened host range, can be concentrated to high titer, and mediate stable transduction of genes into cells. The RRV glycoprotein-pseudotyped retroviruses and the cells that produce them have been employed to demonstrate that RRV glycoprotein-mediated viral entry occurs through endocytosis and that membrane fusion requires acidic pH. Alphavirus glycoprotein-pseudotyped retroviruses have significant advantages as reagents for the study of the biochemistry and prevention of alphavirus entry and as preferred vectors for stable gene transfer and gene therapy protocols.
假型逆转录病毒作为基因转移和基因治疗的载体以及研究病毒糖蛋白功能的工具具有重要应用。基于重组莫洛尼鼠白血病病毒（Mo-MuLV）的逆转录病毒颗粒能有效整合甲病毒罗斯河病毒（RRV）的糖蛋白，并利用它们进入细胞。已经构建了能长时间产生RRV糖蛋白假型逆转录病毒的稳定细胞系。假型病毒具有拓宽的宿主范围，能浓缩至高滴度，并介导基因稳定转导进入细胞。RRV糖蛋白假型逆转录病毒及其产生细胞已被用于证明RRV糖蛋白介导的病毒进入是通过内吞作用发生的，且膜融合需要酸性pH。甲病毒糖蛋白假型逆转录病毒作为研究甲病毒进入的生物化学和预防的试剂以及稳定基因转移和基因治疗方案的首选载体具有显著优势。
