Wool-Lewis R J, Bates P
Department of Microbiology, University of Pennsylvania School of Medicine, Philadelphia 19104-6076, USA.
J Virol. 1998 Apr;72(4):3155-60. doi: 10.1128/JVI.72.4.3155-3160.1998.
Studies analyzing Ebola virus replication have been severely hampered by the extreme pathogenicity of this virus. To permit analysis of the host range and function of the Ebola virus glycoprotein (Ebo-GP), we have developed a system for pseudotyping these glycoproteins into murine leukemia virus (MLV). This pseudotyped virus, MLV(Ebola), can be readily concentrated to titers which exceed 5 x 10(6) infectious units/ml and is effectively neutralized by antibodies specific for Ebo-GP. Analysis of MLV(Ebola) infection revealed that the host range conferred by Ebo-GP is very broad, extending to cells of a variety of species. Notably, all lymphoid cell lines tested were completely resistant to infection; we speculate that this is due to the absence of a cellular receptor for Ebo-GP on B and T cells. The generation of high-titer MLV(Ebola) pseudotypes will be useful for the analysis of immune responses to Ebola virus infection, development of neutralizing antibodies, analysis of glycoprotein function, and isolation of the cellular receptor(s) for the Ebola virus.
对埃博拉病毒复制的研究因该病毒的极端致病性而受到严重阻碍。为了能够分析埃博拉病毒糖蛋白（Ebo-GP）的宿主范围和功能，我们开发了一种将这些糖蛋白伪型化到鼠白血病病毒（MLV）中的系统。这种伪型病毒，即MLV（埃博拉），可以很容易地浓缩到滴度超过5×10⁶感染单位/毫升，并且能被针对Ebo-GP的特异性抗体有效中和。对MLV（埃博拉）感染的分析表明，Ebo-GP赋予的宿主范围非常广泛，延伸到多种物种的细胞。值得注意的是，所有测试的淋巴细胞系对感染完全有抗性；我们推测这是由于B细胞和T细胞上缺乏Ebo-GP的细胞受体。高滴度MLV（埃博拉）伪型的产生将有助于分析对埃博拉病毒感染的免疫反应、中和抗体的开发、糖蛋白功能的分析以及埃博拉病毒细胞受体的分离。
