Weiss M A
Department of Biochemistry Case Western Reserve University Cleveland, Ohio 44106, USA.
Mol Endocrinol. 2001 Mar;15(3):353-62. doi: 10.1210/mend.15.3.0617.
The high-mobility group (HMG) box defines a DNA-bending motif of broad interest in relation to human development and disease. Major and minor wings of an L-shaped structure provide a template for DNA bending. As in the TATA-binding protein and a diverse family of factors, insertion of one or more side chains between base pairs induces a DNA kink. The HMG box binds in the DNA minor groove and may be specific for DNA sequence or distorted DNA architecture. Whereas the angular structures of non-sequence-specific domains are well ordered, free SRY and related autosomal SOX domains are in part disordered. Observations suggesting that the minor wing lacks a fixed tertiary structure motivate the hypothesis that DNA bending and stabilization of protein structure define a coupled process. We further propose that mutual induced fit in SOX-DNA recognition underlies the sequence dependence of DNA bending and enables the induction of promoter-specific architectures.
高迁移率族（HMG）盒定义了一种与人类发育和疾病相关的、广泛关注的DNA弯曲基序。L形结构的主翼和次翼为DNA弯曲提供了模板。与TATA结合蛋白及多种因子家族一样，在碱基对之间插入一个或多个侧链会诱导DNA扭结。HMG盒结合于DNA小沟，可能对DNA序列或扭曲的DNA结构具有特异性。非序列特异性结构域的角状结构排列有序，而游离的SRY及相关常染色体SOX结构域部分无序。表明次翼缺乏固定三级结构的观察结果促使人们提出这样的假说：DNA弯曲和蛋白质结构的稳定定义了一个耦合过程。我们进一步提出，SOX-DNA识别中的相互诱导契合是DNA弯曲序列依赖性的基础，并能够诱导启动子特异性结构。
