Mole L E, Goodfriend L, Lapkoff C B, Kehoe J M, Capra J D
Biochemistry. 1975 Mar 25;14(6):1216-20. doi: 10.1021/bi00677a019.
The complete amino acid sequence of Ra5, a ragweed pollen allergen, has been determined. Allergen Ra5 is a low molecular weight protein of 45 residues derived from Ambrosia elatior, the short ragweed. It contains no detectable carbohydrate or lipid and has four disulfide bridges. The total structure was determined on 1.4 mumol of material and indicates that structural analysis is increasingly possible on relatively small amounts of highly purified material when a combination of automated and manual sequencing techniques and highly sensitive detection systems is employed. This represents the first complete amino acid sequence of a ragweed allergen and it should provide a basis for many structure-function correlative experiments in the field of immediate hypersensitivity.
已确定豚草花粉过敏原Ra5的完整氨基酸序列。过敏原Ra5是一种由矮豚草（Ambrosia elatior）产生的、含有45个残基的低分子量蛋白质。它不含可检测到的碳水化合物或脂质，有四个二硫键。在1.4微摩尔该物质上确定了其整体结构，这表明当采用自动和手动测序技术以及高度灵敏的检测系统相结合的方法时，对相对少量的高纯度物质进行结构分析越来越可行。这是豚草过敏原的首个完整氨基酸序列，应为速发型超敏反应领域的许多结构-功能相关实验提供基础。
