Suzuki T, Sasano H, Kaneko C, Ogawa S, Darnel A D, Krozowski Z S
Department of Pathology, Tohoku University School of Medicine, 2-1 Seiryo-machi, Aoba-ku, 980-8575, Sendai, Japan.
Mol Cell Endocrinol. 2001 Feb 28;173(1-2):121-5. doi: 10.1016/s0303-7207(00)00403-2.
11beta-hydroxysteroid dehydrogenase (11beta-HSD) regulates local actions of corticosteroids at glucocorticoid and mineralocorticoid receptors. Corticosteroids are thought to play important roles in ocular function. However, mechanisms of intraocular corticosteroid action are still unclear. Therefore, in this study, we examined the immunohistochemical localization of 11beta-HSD type 1 (11beta-HSD1), 11beta-HSD type 2 (11beta-HSD2), mineralocorticoid receptor (MR) and glucocorticoid receptor (GR) in human ocular tissues from patients (6 months to 78 years of age; n = 10) retrieved from surgical pathology files. Both 11beta-HSD2 and MR immunoreactivity was detected only in non-pigmented epithelium of the ciliary body, but was undetectable in cornea, lens, iris, retina, choroid and sclera, in all the cases examined. GR was detected in all cell types in the human eye. 11beta-HSD1 immunoreactivity was not detected in the human eye in this study. These results suggest that 11beta-HSD2 play an important role in human ocular mineralocorticoid action, such as the production of aqueous humor, in the ciliary body. The widespread expression of GR suggests that glucocorticoids may play an important role in the function and homeostasis of the human eye.
11β-羟基类固醇脱氢酶（11β-HSD）调节皮质类固醇在糖皮质激素和盐皮质激素受体处的局部作用。皮质类固醇被认为在眼功能中起重要作用。然而，眼内皮质类固醇作用的机制仍不清楚。因此，在本研究中，我们检查了从手术病理档案中获取的患者（6个月至78岁；n = 10）人眼组织中1型11β-羟基类固醇脱氢酶（11β-HSD1）、2型11β-羟基类固醇脱氢酶（11β-HSD2）、盐皮质激素受体（MR）和糖皮质激素受体（GR）的免疫组织化学定位。在所有检查的病例中，仅在睫状体的无色素上皮中检测到11β-HSD2和MR免疫反应性，而在角膜、晶状体、虹膜、视网膜、脉络膜和巩膜中未检测到。在人眼中的所有细胞类型中均检测到GR。在本研究中，人眼中未检测到11β-HSD1免疫反应性。这些结果表明，11β-HSD2在人眼盐皮质激素作用中起重要作用，如在睫状体中产生房水。GR的广泛表达表明糖皮质激素可能在人眼的功能和内环境稳定中起重要作用。
