Suzuki T, Sasano H, Suzuki S, Hirasawa G, Takeyama J, Muramatsu Y, Date F, Nagura H, Krozowski Z S
Department of Pathology, Tohoku University School of Medicine, Sendai, Japan.
J Clin Endocrinol Metab. 1998 Nov;83(11):4022-5. doi: 10.1210/jcem.83.11.5227.
11Beta-hydroxysteroid dehydrogenase type 2 (11betaHSD2) catalyzes the conversion of cortisol to biologically inactive cortisone and is thought to confer specificity on mineralocorticoid receptors (MR). Cortisol is a prerequisite for surfactant synthesis and fetal lung maturation. Recently, expression of 11betaHSD2 was demonstrated in human fetal lung, but its localization and possible biological roles remain unknown. Therefore, in this study, we examined immunohistochemical localization of 11betaHSD2, MR, and glucocorticoid receptor (GR) in nonpathological human lungs from fetus to adult (8 weeks gestation to 55 yr of age; n = 40) retrieved from pathology files. Both 11betaHSD2 and MR immunoreactivities were detected in airway epithelia, from bronchiole to trachea and in fetal and neonatal ciliated collecting duct cells of tracheal and bronchial glands, but were undetectable in alveoli. On the other hand, GR was detected in all cell types. These results indicate that 11betaHSD2 colocalizes with MR in human airway epithelia and suggest that 11betaHSD2 play an important role in pulmonary mineralocorticoid activity such as sodium and fluid transport.
11β-羟类固醇脱氢酶2型（11βHSD2）催化皮质醇转化为生物活性较低的可的松，被认为赋予盐皮质激素受体（MR）特异性。皮质醇是表面活性剂合成和胎儿肺成熟的前提条件。最近，在人胎儿肺中证实了11βHSD2的表达，但其定位和可能的生物学作用尚不清楚。因此，在本研究中，我们从病理学档案中检索了从胎儿到成人（妊娠8周龄至55岁；n = 40）的非病理性人肺组织，检测了11βHSD2、MR和糖皮质激素受体（GR）的免疫组织化学定位。在从细支气管到气管的气道上皮以及气管和支气管腺体的胎儿和新生儿纤毛集合管细胞中均检测到11βHSD2和MR免疫反应性，但在肺泡中未检测到。另一方面，在所有细胞类型中均检测到GR。这些结果表明，11βHSD2与人气道上皮中的MR共定位，并提示11βHSD2在肺盐皮质激素活性（如钠和液体转运）中起重要作用。
