Meyer R A, Brunsting A
Biophys J. 1975 Mar;15(3):191-203. doi: 10.1016/S0006-3495(75)85811-5.
The light scattered from nucleated biological cells has been investigated by using four different theoretical models: an opaque disk, a homogeneous sphere, an opaque ring, and a coated sphere. By comparing these four models, diffraction at the edges of the cell and the nucleus has been found to be the predominate scattering mechanism for nucleated biological cells at low angles. The scattering patterns of nucleated cells are found to have a fine lobe (high-frequency) structure dependent on whole cell size, and an envelope lobe (low-frequency) structure dependent on relative nucleus size. The models indicate that the present technique for measuring cell size with a single low-angle light detector is highly dependent on the nucleus to cell diameter ratio. Whole cell size is better estimated by the ratio of the outputs from two low-angle detectors.
不透明圆盘、均匀球体、不透明环和包覆球体。通过比较这四种模型，发现在低角度下，细胞和细胞核边缘的衍射是有核生物细胞的主要散射机制。有核细胞的散射图案具有依赖于整个细胞大小的精细叶瓣（高频）结构和依赖于相对细胞核大小的包络叶瓣（低频）结构。这些模型表明，目前使用单个低角度光探测器测量细胞大小的技术高度依赖于细胞核与细胞直径的比率。通过两个低角度探测器输出的比率可以更好地估计整个细胞的大小。
