Arndt D, Parwaresch M R, Bodenstein H
Blut. 1975 Mar;30(3):199-202. doi: 10.1007/BF01633954.
A new technique is described which enables a selective tinctorial visualization of human blood basophils in autoradiographic preparations. Consecutive administration of 5-aminoacridine hydrochloride and aldehyde fuchsin rendered a sufficient preservation of basophils preventing leaching of their water soluble granules. Extensive control tests provided sufficient evidence to exclude chemographic artifacts produced by other histochemical precedures. The technique eliminates the obstacles to study the kinetics of human blood basophils and their realtionshp to atopic hypersensitivity reactions applying tritiated thymidine and radioiodine labeled IgE or corresponding antisera.
本文描述了一种新技术，该技术能够在放射自显影片中对人血嗜碱性粒细胞进行选择性染色显示。连续给予盐酸5-氨基吖啶和醛复红可充分保存嗜碱性粒细胞，防止其水溶性颗粒被浸出。大量对照试验提供了充分证据，排除了其他组织化学方法产生的化学假象。该技术消除了研究人血嗜碱性粒细胞动力学及其与特应性超敏反应关系的障碍，可应用氚标记胸腺嘧啶核苷和放射性碘标记的IgE或相应抗血清。
