Parwaresch M R, Arndt D
Histochem J. 1975 Jul;7(4):357-60. doi: 10.1007/BF01007020.
The aim of this paper is the selective visualization of human blood basophils in autoradiographs. [(3)H]Thymidine-labelled basophils in buffy coat smears were fixed in methanol-formaldehyde follwed by 5-aminoacridine hydrochloride and stained with basic Aldehyde Fuchsin prior to autoradiographic processing. The technique described represents a simple method for quantitative autoradiographic studies on basophil kinetics and the interaction of these cells with IgE-mediated atopias.
本文的目的是在放射自显影片中对人类血液嗜碱性粒细胞进行选择性可视化。将血沉棕黄层涂片上用[³H]胸腺嘧啶核苷标记的嗜碱性粒细胞先用甲醇 - 甲醛固定，接着用盐酸5 - 氨基吖啶处理，然后在进行放射自显影处理之前用碱性醛品红染色。所描述的技术是一种用于嗜碱性粒细胞动力学以及这些细胞与IgE介导的特应性疾病相互作用的定量放射自显影研究的简单方法。
