Xue Y, Smedts F, Ruijter E T, Debruyne F M, de la Rosette J J, Schalken J A
Department of Urology, University Hospital Nijmegen, The Netherlands.
Eur Urol. 2001 Feb;39(2):222-31. doi: 10.1159/000052440.
Knowledge regarding cell biologic characteristics of small solid glandular buds in the prostate and their relationship with branching activity in the human prostate is still fragmentary. Our object was to demonstrate, on the basis of immunophenotype, loci that harbor the potential for branching activity within the adult human prostate.
Semiserial sectioning was performed on 13 adult prostates in an effort to identify structures in the prostate that could be considered foci of growth. Selected slides were stained with biomarkers for basal/luminal cells (keratins), proliferation (MIB-1), apoptosis inhibitor (bcl-2), intercellular adhesion (E-cadherin), and stromal-epithelial interactions (tenascin-C). Results were compared with fetal and prepubertal human prostates and microdissected rat prostates.
Five histologic epithelial structures were identified in 19 paraffin blocks, which on serial sectioning showed morphologic transitions with a common pattern, consisting of reduction in number and caliber of acini until small solid buds of epithelial cells were reached. Immunophenotypically, the small solid glandular buds had a basal-cell keratin phenotype, expression of bcl-2 in virtually all cells, high proliferative activity, prominent intracellular localization of E-cadherin, and enhanced periglandular tenascin-C immunoreactivity. The budding tips in fetal and prepubertal prostates revealed an immunostaining pattern identical to the small solid glandular buds in the adult, but different to the rat prostate.
Our data suggest that dispersed small solid glandular buds have a capacity for growth, and as such may be considered foci of resumed reawakening branching activity with in the adult human prostate.
关于前列腺中小的实性腺芽的细胞生物学特性及其与人前列腺中分支活动的关系的知识仍然支离破碎。我们的目的是基于免疫表型来证明成年男性前列腺内具有分支活动潜力的位点。
对13个成人前列腺进行半连续切片，以识别前列腺中可被视为生长灶的结构。选择的玻片用基底/管腔细胞（角蛋白）、增殖（MIB-1）、凋亡抑制剂（bcl-2）、细胞间粘附（E-钙粘蛋白）和基质-上皮相互作用（腱生蛋白-C）的生物标志物进行染色。将结果与胎儿和青春期前的人类前列腺以及显微切割的大鼠前列腺进行比较。
在19个石蜡块中识别出5种组织学上皮结构，连续切片显示出具有共同模式的形态转变，包括腺泡数量和管径减少，直至达到上皮细胞的小实性芽。免疫表型上，小实性腺芽具有基底细胞角蛋白表型，几乎所有细胞中都有bcl-2表达，高增殖活性，E-钙粘蛋白在细胞内显著定位，以及腺周腱生蛋白-C免疫反应性增强。胎儿和青春期前前列腺中的芽尖显示出与成人小实性腺芽相同的免疫染色模式，但与大鼠前列腺不同。
我们的数据表明，分散的小实性腺芽具有生长能力，因此可被视为成年男性前列腺内重新恢复分支活动的灶点。
