Shiraishi T, Kato H, Komada S, Imai H, Hirokawa Y, Kusano I, Yatani R, Sakakura T
Department of Pathology, Mie Univeristy School of Medicine, Japan.
Int J Dev Biol. 1994 Jun;38(2):391-5.
We investigated the distribution of tenascin during postnatal development of the human prostate. Monoclonal antibody specific to human tenascin was applied to paraffin sections by the avidin-biotin-complex method to examine its localization. Infantile prostates showed two distinct zones. The inner zone had sparse acini with fibromuscular bundles. The peripheral zone had similar acini and lighter stroma as compared with inner zone. Tenascin was found diffusely but weakly. The periglandular area occasionally showed immunoreactivity. The prostates from 9- to 13-year-old subjects had a morphology similar to that of the infantile gland. The difference was increased density of the acini. Immunoreactivity was low. In the prostates from 14- to 21-year-old subjects, the acini distribution was more crowded, and the epithelial lining had become taller in both zones. Tenascin distributed preferentially in the peripheral zone during this period. Simultaneously, the percent of glandular area in the peripheral zone rose abruptly. The dynamics of tenascin expression are closely associated with the development and maturation of the gland. The distribution of tenascin during the post-puberal period may suggest its participation in the preferential of prostatic carcinoma in the peripheral zone.
我们研究了腱生蛋白在人前列腺出生后发育过程中的分布情况。通过抗生物素蛋白-生物素复合物法将特异性针对人腱生蛋白的单克隆抗体应用于石蜡切片，以检查其定位。婴儿前列腺呈现出两个不同的区域。内部区域有稀疏的腺泡和纤维肌束。与内部区域相比，周边区域有类似的腺泡且间质较浅。腱生蛋白呈弥漫性但弱阳性表达。腺周区域偶尔显示免疫反应性。9至13岁受试者的前列腺形态与婴儿期腺体相似。不同之处在于腺泡密度增加。免疫反应性较低。在14至21岁受试者的前列腺中，腺泡分布更密集，两个区域的上皮衬里都变高了。在此期间，腱生蛋白优先分布于周边区域。同时，周边区域腺泡面积的百分比急剧上升。腱生蛋白表达的动态变化与腺体的发育和成熟密切相关。青春期后腱生蛋白的分布可能表明其参与了前列腺癌在周边区域的偏好发生。
