Kaul A, Kalla N R, Goyle S
Human Molecular Genetics Laboratory, School of Life Sciences, Jawaharlal Nehru University, New Delhi-110067, India.
Teratog Carcinog Mutagen. 2001;21(2):151-64.
Lymphocyte proliferation kinetics (LPK) is an end point used in genetic toxicology that was proposed as an alternative for the screening of anticonvulsant drugs. The effect of phenytoin (PHT) was investigated on the mitotic and proliferation indices in cultured blood lymphocytes of 33 sporadically collected untreated and 42 PHT-treated epileptics, where the duration of treatment was 3, 6, and 9 months, and 40 control subjects (age range 10-30 years). PHT induced mitotic delays and decreased the mitotic index. A significant heterogeneity of the first, second and the third metaphases between treated and untreated groups was revealed. A reduction of the proliferation index (P < 0.001) and proliferation delay per cycle (P < 0.001) was also observed. There was little variation between the controls and untreated patients (P > 0.05). The results have confirmed that PHT can affect responses leading to genotoxicity. Teratogenesis Carcinog. Mutagen. 21:151-164, 2001.
淋巴细胞增殖动力学（LPK）是遗传毒理学中使用的一个终点指标，被提议作为筛选抗惊厥药物的替代方法。研究了苯妥英（PHT）对33例偶尔收集的未经治疗的癫痫患者以及42例接受PHT治疗3、6和9个月的癫痫患者和40名对照受试者（年龄范围10 - 30岁）培养的血液淋巴细胞有丝分裂和增殖指数的影响。PHT诱导有丝分裂延迟并降低有丝分裂指数。在治疗组和未治疗组之间，第一、第二和第三中期存在显著异质性。还观察到增殖指数降低（P < 0.001）和每个周期的增殖延迟（P < 0.001）。对照组和未治疗患者之间差异不大（P > 0.05）。结果证实PHT可影响导致遗传毒性的反应。《致畸、致癌、致突变》2001年第21卷第151 - 164页
