Siviková K
Department of Veterinary Genetics, University of Veterinary Medicine, Kosice, Slovak Republic.
Folia Biol (Praha). 1996;42(3):87-91.
The mitotic (MI) and proliferation (PI) indices were investigated to determine the effect of N-nitrosodimethylamine (NDMA) in cultured blood lymphocytes of cows, pigs, and sheep. Our results confirmed the ability of all animal lymphocytes to metabolize this chemical agent to mutagenic products. Treatment with NDMA caused inhibition of MI and PI which were more expressive in bovine lymphocytes. In addition, statistically significant heterogeneity of the first, second, and third metaphases between the treated and untreated cultures was found. Mean PI values were used to estimate cellcycle delays showing the highest effect in bovine lymphocytes.
研究有丝分裂指数（MI）和增殖指数（PI），以确定N-亚硝基二甲胺（NDMA）对牛、猪和羊培养的血液淋巴细胞的影响。我们的结果证实了所有动物淋巴细胞将这种化学物质代谢为诱变产物的能力。用NDMA处理会导致MI和PI受到抑制，这在牛淋巴细胞中表现得更为明显。此外，在处理过的和未处理的培养物之间，发现第一、第二和第三中期存在统计学上显著的异质性。平均PI值用于估计细胞周期延迟，结果表明其对牛淋巴细胞的影响最大。
