Plumb R S, Warwick H, Higton D, Dear G J, Mallett D N
Glaxo Wellcome Research and Development, BioMet Division, Park Road, Ware, Herts SG12 ODP, UK.
Rapid Commun Mass Spectrom. 2001;15(4):297-303. doi: 10.1002/rcm.225.
Capillary high-performance liquid chromatography (HPLC; 300 microm i.d.) coupled to tandem mass spectrometry has been used to determine the concentration of 4-hydroxytamoxifen in mouse plasma in the pg/mL range following the administration of Tamoxifen. A limit of quantification (LOQ) of 100 pg/mL was achieved using only 25 microL of plasma. The on-column sensitivity was determined to be 100 fg. The column performance was determined isocratically before and after the assay and showed only a 15% reduction in performance after 70 injections of plasma extract. No significant peak band broadening was observed due to the mass spectrometer interface using a standard TurboIonspray source.
毛细管高效液相色谱法（HPLC；内径300微米）与串联质谱联用，用于测定给予他莫昔芬后小鼠血浆中4-羟基他莫昔芬的浓度，范围为皮克/毫升。仅使用25微升血浆就实现了100皮克/毫升的定量限（LOQ）。柱上灵敏度测定为100飞克。在测定前后等度测定柱性能，在注射70次血浆提取物后，性能仅降低了15%。使用标准TurboIonspray源的质谱仪接口未观察到明显的峰带展宽。
