Center for the Study of Animal Science, ICETA, University of Porto.
Metabolism. 2013 Sep;62(9):1206-17. doi: 10.1016/j.metabol.2013.04.003. Epub 2013 May 7.
Mass spectrometric approaches have been fundamental to the identification of metabolites associated with steroid hormones, yet this topic has not been reviewed in depth in recent years. To this end, and given the increasing relevance of liquid chromatography-mass spectrometry (LC-MS) studies on steroid hormones and their metabolites, the present review addresses this subject. This review provides a timely summary of the use of various mass spectrometry-based analytical techniques during the evaluation of steroidal biomarkers in a range of human disease settings. The sensitivity and specificity of these technologies are clearly providing valuable new insights into breast cancer and cardiovascular disease. We aim to contribute to an enhanced understanding of steroid metabolism and how it can be profiled by LC-MS techniques.
质谱分析方法对于鉴定与甾体激素相关的代谢物至关重要，但近年来这一主题并未得到深入综述。有鉴于此，并且鉴于液相色谱-质谱（LC-MS）研究在甾体激素及其代谢物方面的相关性日益增强，本综述针对该主题展开讨论。本综述及时总结了在各种人类疾病环境下评估甾体生物标志物时使用各种基于质谱的分析技术的情况。这些技术的灵敏度和特异性显然为乳腺癌和心血管疾病提供了有价值的新见解。我们旨在增进对甾体代谢及其如何通过 LC-MS 技术进行分析的理解。
