Cherek D.R., Bennett R.H., Roache J.D., Rose J.E.
Human Behavioral Pharmacology Laboratory/Substance Abuse Research Center, Department of Psychiatry and Behavioral Sciences, University of Texas Health Science Center at Houston, USA.
Behav Pharmacol. 1991 Feb;2(1):15-22.
A previously developed spirometric methodology of tobacco smoke administration was evaluated by determining the effects of varying nicotine delivery on various physiological and subjective measures. Eight male tobacco smoking subjects were administered 60cc volumes of tobacco smoke drawn from University of Kentucky research cigarettes, or air. Subjects were exposed to four bouts of smoke administration conducted over an 8h day. Each smoking bout was separated by 2h and involved 20 smoke administrations at the rate of one every 30sec. Each smoke administration consisted of 60cc of air or 60cc drawn from 0.3, 1.2 or 2.7mg nicotine yield cigarettes, followed by 1 liter of air which forced the smoke or air deep into the lungs. Carbon monoxide (CO), blood pressure, and heart rate were measured before and after each smoking bout, and subject ratings of smoke effects were completed after each smoking bout. In a separate study, blood samples were collected on two occasions before and after administration of the two highest nicotine yield cigarettes to determine changes in nicotine plasma levels. Data indicated that the spirometric method produced: (1) similar CO boosts across nicotine yields, and (2) changes in heart rate, blood pressure, subject ratings and plasma nicotine levels which were directly related to the nicotine yield of cigarettes.
通过确定不同尼古丁输送量对各种生理和主观指标的影响，对先前开发的烟草烟雾吸入肺活量测定方法进行了评估。八名男性吸烟受试者吸入了从肯塔基大学研究用香烟中抽取的60立方厘米烟草烟雾或空气。受试者在8小时内接受了四轮烟雾吸入。每轮吸烟间隔2小时，以每30秒一次的速率进行20次烟雾吸入。每次烟雾吸入包括60立方厘米空气或从尼古丁产率为0.3、1.2或2.7毫克的香烟中抽取的60立方厘米烟雾，随后是1升空气，将烟雾或空气压入肺部深处。在每次吸烟前后测量一氧化碳（CO）、血压和心率，并在每次吸烟后完成受试者对烟雾影响的评分。在另一项研究中，在吸入两种尼古丁产率最高的香烟前后两次采集血样，以确定血浆尼古丁水平的变化。数据表明，肺活量测定法产生了：（1）不同尼古丁产率下类似的一氧化碳增加量，以及（2）心率、血压、受试者评分和血浆尼古丁水平的变化，这些变化与香烟的尼古丁产率直接相关。
