de Beun R., Heinsbroek R.P.W., Slangen J.L., van De Poll N.E.
Netherlands Institute for Brain Research, Meibergdreef 33, 1105 AZ Amsterdam, The Netherlands State University of Utrecht, Faculty of Pharmacy, Sorbonnelaan 16, 3584 CA Utrecht, The Netherlands.
Behav Pharmacol. 1991 Dec;2(6):439-445.
Gonadectomized male and female rats were trained to discriminate 50µg/kg estradiol-17B(E(2)) from its vehicle in a one-bottle forced-drinking discriminative taste-aversion procedure. The animals were injected SC with E(2) or arachidic oil, 60min prior to daily training sessions during which they had access to a saccharin (Sac) solution for 10min. Animals injected with E(2) prior to Sac-LiCl pairings and with arachidic oil prior to Sac-NaCl pairings acquired hormone discrimination, only suppressing Sac intake following the administration of E(2) and not following the administration of arachidic oil. Both males and females reliably discriminated E(2) from arachidic oil within 28 training sessions. Subsequent generalization tests revealed dose-dependent stimulus control of Sac intake by E(2) in both sexes (range of substitution doses: 0.4-250µg/kg). The results show that E(2) may act as a discriminative stimulus in male and female rats.
对去性腺的雄性和雌性大鼠进行训练，使其在单瓶强制饮水辨别味觉厌恶程序中区分50μg/kg的17β-雌二醇（E(2)）与其溶剂。在每日训练前60分钟，给动物皮下注射E(2)或花生油，训练期间动物可接触糖精（Sac）溶液10分钟。在Sac-LiCl配对前注射E(2)且在Sac-NaCl配对前注射花生油的动物获得了激素辨别能力，仅在注射E(2)后而非注射花生油后抑制Sac摄入。雄性和雌性在28次训练内均可靠地区分了E(2)和花生油。随后的泛化测试显示，E(2)对两性的Sac摄入具有剂量依赖性的刺激控制作用（替代剂量范围：0.4-250μg/kg）。结果表明，E(2)在雄性和雌性大鼠中可能作为一种辨别性刺激物。
