Pérez V, Pastó M, Unzeta M
Departament de Bioquímica i de Biologia Molecular, Facultat de Medicina, Universitat Autònoma de Barcelona, 08193 Bellalterra, Spain.
Neurobiology (Bp). 2000;8(3-4):237-42.
Rat striatal slices were incubated 1 hour in Krebs buffer with carbogen by continuous perfusion. When both MAO-B inhibitors PF9601N and l-deprenyl were added in the incubation medium, at 200 microM concentration, a diminution in the dopamine content was observed. The decrease in dopamine content was partially calcium dependent and showed a different mechanism between both compounds. When 1 mM concentration EGTA was added at the Krebs incubation medium without calcium, dopamine content was partially recovered, being not affected in case of l-deprenyl. When 50 microM dantrolene (a calcium vesicular release inhibitor) was added in the Krebs incubation medium without calcium, dopamine was partially recovered for l-deprenyl, being not affected in case of PF9601N. These data indicate that dopamine release is extracellular calcium dependent in case of PF9601N, whereas l-deprenyl depends on calcium intracellular origin.
大鼠纹状体切片在含二氧化碳的Krebs缓冲液中通过连续灌注孵育1小时。当在孵育培养基中加入MAO - B抑制剂PF9601N和l - 司来吉兰，浓度为200微摩尔时，观察到多巴胺含量降低。多巴胺含量的降低部分依赖于钙，且两种化合物之间显示出不同的机制。当在不含钙的Krebs孵育培养基中加入1毫摩尔浓度的乙二醇双（2 - 氨基乙基醚）四乙酸（EGTA）时，多巴胺含量部分恢复，而l - 司来吉兰的情况不受影响。当在不含钙的Krebs孵育培养基中加入50微摩尔丹曲林（一种钙囊泡释放抑制剂）时，l - 司来吉兰的多巴胺部分恢复，PF9601N的情况不受影响。这些数据表明，PF9601N情况下多巴胺释放依赖于细胞外钙，而l - 司来吉兰依赖于细胞内钙来源。
