脑室内注射促肾上腺皮质激素释放因子在小鼠中以非常低的剂量就会抑制食物摄入并产生类似焦虑的行为。
Intracerebroventricularly administered corticotropin-releasing factor inhibits food intake and produces anxiety-like behaviour at very low doses in mice.
作者信息
Momose K, Inui A, Asakawa A, Ueno N, Nakajima M, Fujimiya M, Kasuga M
机构信息
Metabolic Disease Research, Pharmacology Laboratories, Institute for Drug Discovery Research, Yamanouchi Pharmaceutical Co. Ltd, Tsukuba, Japan.
出版信息
Diabetes Obes Metab. 1999 Sep;1(5):281-4. doi: 10.1046/j.1463-1326.1999.00033.x.
AIM
Previous studies have demonstrated that corticotropin-releasing factor (CRF) produces behavioural, physiological and immunological responses similar to those induced by stress. However, these findings have been validated largely in laboratory rats.
METHODS
We examined the effects of intracerebroventricular (i.c.v.) administration of CRF on anxiety and food intake in mice. Using the elevated-plus maze, we measured anxiety levels after i.c.v. CRF in mice. We also measured food intake for 2 h after i.c.v. CRF.
RESULTS
CRF increased the normal preference for the closed arms of the maze at a very low dose of 3 pmol, indicating an anxiogenic effect. CRF powerfully suppressed food intake at the doses of 3-300 pmol for over 2 h.
CONCLUSION
Our results demonstrate that i.c.v. CRF evokes anxiogenic behaviour and suppresses feeding with the same dose-response relationships in mice. CRF may thus play a role in integrating the overall responses to stress through co-ordinated actions in the brain of this species.
目的
先前的研究表明，促肾上腺皮质激素释放因子（CRF）产生的行为、生理和免疫反应与应激诱导的反应相似。然而，这些发现主要在实验室大鼠中得到验证。
方法
我们研究了脑室内（i.c.v.）注射CRF对小鼠焦虑和食物摄入的影响。使用高架十字迷宫，我们测量了i.c.v.注射CRF后小鼠的焦虑水平。我们还测量了i.c.v.注射CRF后2小时内的食物摄入量。
结果
CRF在非常低的剂量3 pmol时增加了对迷宫封闭臂的正常偏好，表明具有致焦虑作用。CRF在3 - 300 pmol的剂量下强力抑制食物摄入超过2小时。
结论
我们的结果表明，i.c.v.注射CRF在小鼠中引发致焦虑行为并以相同的剂量反应关系抑制进食。因此，CRF可能通过在该物种大脑中的协同作用，在整合对应激的整体反应中发挥作用。
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