Furuta H, Fujihara N
Division of Bioresource and Bioenvironmental Sciences, Graduate School Kyushu University, Fukuoka, Japan.
Asian J Androl. 1999 Dec;1(4):187-90.
This study was designed to investigate the effect of busulfan treatment on the proliferation of chicken primordial germ cells (PGCs) in vivo, focusing on the preferential settlement of PGCs onto the germinal ridges of chicken embryos.
Busulfan (250 ng/egg) was injected into the egg white of freshly oviposited fertilized eggs, which were then incubated. Embryonic development and viability were examined, and exogenous PGCs collected from embryonic blood vessels were injected into the germinal crescent region of recipient embryos. The number of PGCs resided onto germinal ridges of the right and left sides were compared.
Busulfan had a slight harmful effect on the embryo viability and the PGCs proliferation. The number of PGCs resided onto the left side of germinal ridges was slightly higher as compared with the right side.
Busulfan suppressed the viability of embryos and the proliferation of endogenous PGCs in the recipient embryos. However, the number of exogenous PGCs proliferated was higher in embryos treated with busulfan than those without busulfan. Data also suggest the possibility of a preferential residence of PGCs toward the left side of the germinal crescent region as compared with the right, which may be due to a more advanced functional development of the left gonad than the right.
本研究旨在探讨白消安处理对鸡原始生殖细胞（PGCs）体内增殖的影响，重点关注PGCs在鸡胚生殖嵴上的优先定居情况。
将白消安（250 ng/蛋）注射到刚产出的受精卵的蛋清中，然后进行孵化。检查胚胎发育和活力，并将从胚胎血管中收集的外源性PGCs注射到受体胚胎的生殖新月区。比较左右两侧生殖嵴上定居的PGCs数量。
白消安对胚胎活力和PGCs增殖有轻微有害影响。与右侧相比，定居在左侧生殖嵴上的PGCs数量略高。
白消安抑制了受体胚胎的活力和内源性PGCs的增殖。然而，用白消安处理的胚胎中外源性PGCs增殖的数量高于未用白消安处理的胚胎。数据还表明，与右侧相比，PGCs可能更倾向于在生殖新月区左侧定居，这可能是由于左侧性腺的功能发育比右侧更先进。
