Chang I K, Jeong D K, Hong Y H, Park T S, Moon Y K, Ohno T, Han J Y
Department of Animal Science and Technology, College of Agriculture and Life Sciences, Seoul National University, Suweon, Korea.
Cell Biol Int. 1997 Aug;21(8):495-9. doi: 10.1006/cbir.1997.0173.
Primordial germ cells (PGCs) from stage 27 (5.5-day-old) Korean native ogol chicken embryonic germinal ridges were cultured in vitro for 5 days. As in in vivo culture, these cultured PGCs were expected to have already passed beyond the migration stage. Approximately 200 of these PGCs were transferred into 2.5-day-old white leghorn embryonic blood stream, and then the recipient embryos were incubated until hatching. The rate of hatching was 58.8% in the manipulated eggs. Six out of 60 recipients were identified as germline chimeric chickens by their feather colour. The frequency of germline transmission of donor PGCs was 1.3-3.1% regardless of sex. The stage 27 PGCs will be very useful for collecting large numbers of PGCs, handling of exogenous DNA transfection during culture, and for the production of desired transgenic chickens.
取自27期（5.5日龄）韩国本地五黑鸡胚胎生殖嵴的原始生殖细胞（PGCs）在体外培养5天。与体内培养一样，这些培养的PGCs预计已经越过迁移阶段。将大约200个这些PGCs转移到2.5日龄白来航鸡胚胎血流中，然后将受体胚胎孵化至出雏。处理过的种蛋出雏率为58.8%。60只受体中有6只通过羽毛颜色被鉴定为种系嵌合鸡。无论性别，供体PGCs的种系传递频率为1.3 - 3.1%。27期的PGCs对于收集大量PGCs、培养期间处理外源DNA转染以及生产所需的转基因鸡将非常有用。
