Wen R Q, Li S Q, Wang C X, Wang Q H, Liu M Y
Family Planning Research Institute of Guangdong, Guangzhou, China.
Asian J Androl. 1999 Jun;1(1-2):61-5.
To investigate the morphological changes of spermatozoa in the proximal vas deferens after vasectomy.
Proximal vas deferens fluids were collected from 79 fertile men (group A) and 64 vasectomized men (group B) during the operations of vasectomy or vasovasostomy. Sperm morphology in the proximal vas deferens was analyzed after staining with the modified Papanicolaou method.
The percentage of spermatozoa with a normal oval head from group B (50. 7% +/- 21.7%) was significantly lower than that of group A (75.2% +/- 11.1%). The data in group A was similar to those of normal semen and therefore represents the physiological condition of the proximal vas deferens sperm of fertile men. There were no significant differences in the percentages of normal oval heads in group B with the time since vasectomy.
After vasectomy, the spermatozoa in the proximal vas deferens and epididymis were continuously degenerating and being replenished by spermatozoa coming from testis. The obvious morphological degeneration occurred in the sperm tail and spermatozoa with a small oval head and amorphous heads were increased.
研究输精管结扎术后近端输精管内精子的形态变化。
在输精管结扎术或输精管吻合术手术过程中,从79名有生育能力的男性(A组)和64名输精管结扎男性(B组)收集近端输精管液。用改良巴氏染色法染色后分析近端输精管内的精子形态。
B组正常椭圆形头部精子的百分比(50.7%±21.7%)显著低于A组(75.2%±11.1%)。A组的数据与正常精液的数据相似,因此代表了有生育能力男性近端输精管精子的生理状况。B组正常椭圆形头部的百分比与输精管结扎后的时间无显著差异。
输精管结扎术后,近端输精管和附睾内的精子不断退化,并由来自睾丸的精子补充。精子尾部出现明显的形态退化,小椭圆形头部和无定形头部的精子增加。