对生育男性近端输精管精子的分析。

Analysis of spermatozoa from the proximal vas deferens of fertile men.

作者信息

Wen R Q, Li S Q, Wang C X, Wang Q H, Li Q K, Feng H M, Jiang Y J, Zhang X L

机构信息

Family Planning Research Institute, Guangdong, China.

出版信息

Int J Androl. 1993 Apr;16(2):87-91. doi: 10.1111/j.1365-2605.1993.tb01159.x.

DOI:10.1111/j.1365-2605.1993.tb01159.x

PMID:8514432

Abstract

Fluids from the left and right proximal vas deferens were collected from 105 normal fertile men by cannulating the vas deferens during vasectomy, and sperm parameters analysed. Sperm motility (73.1 +/- 13.3%), normal sperm morphology (75.2 +/- 11.1%), sperm viability (72.7 +/- 18.8%) and the hypo-osmotic swelling test (73.3 +/- 19.2%) were in the normal range, compared with that of ejaculated spermatozoa. However, sperm concentration in the proximal vas deferens (6274.6 +/- 5103.8 x 10(6) ml-1 was higher than that in semen. Sperm concentration in the right vas deferens was significantly higher (P < 0.05) than that in the left and the percentage of spermatozoa showing abnormal cervical mucus penetration was significantly higher (47%) for the left than for the right (18%). There were no anti-sperm antibodies on the surface of spermatozoa from the vas deferens as determined by the sperm cervical mucus contact test and immunobead test. These parameters of spermatozoa from the proximal vas may reflect those of spermatozoa from the human cauda epididymis.

摘要

在输精管结扎术中，通过插管从105名正常生育男性的左右近端输精管采集液体，并分析精子参数。与射出精子相比，精子活力（73.1±13.3%）、正常精子形态（75.2±11.1%）、精子存活率（72.7±18.8%）和低渗肿胀试验（73.3±19.2%）均在正常范围内。然而，近端输精管中的精子浓度（6274.6±5103.8×10⁶/ml⁻¹）高于精液中的精子浓度。右侧输精管中的精子浓度显著高于左侧（P<0.05），左侧精子穿透宫颈黏液异常的百分比（47%）显著高于右侧（18%）。通过精子-宫颈黏液接触试验和免疫珠试验确定，输精管精子表面没有抗精子抗体。近端输精管精子的这些参数可能反映了人类附睾尾部精子的参数。