Chiang L W, Grenier J M, Ettwiller L, Jenkins L P, Ficenec D, Martin J, Jin F, DiStefano P S, Wood A
Millennium Pharmaceuticals, 640 Memorial Drive, Cambridge, MA 02139, USA.
Proc Natl Acad Sci U S A. 2001 Feb 27;98(5):2814-9. doi: 10.1073/pnas.051630598.
Programmed cell death (PCD) during neuronal development and disease has been shown to require de novo RNA synthesis. However, the time course and regulation of target genes is poorly understood. By using a brain-biased array of over 7,500 cDNAs, we profiled this gene expression component of PCD in cerebellar granule neurons challenged separately by potassium withdrawal, combined potassium and serum withdrawal, and kainic acid administration. We found that hundreds of genes were significantly regulated in discreet waves including known genes whose protein products are involved in PCD. A restricted set of genes was regulated by all models, providing evidence that signals inducing PCD can regulate large assemblages of genes (of which a restricted subset may be shared in multiple pathways).
已表明神经元发育和疾病过程中的程序性细胞死亡（PCD）需要从头合成RNA。然而，对靶基因的时间进程和调控了解甚少。通过使用包含7500多个cDNA的脑偏向阵列，我们对分别受到钾离子撤除、钾离子和血清联合撤除以及给予 kainic 酸刺激的小脑颗粒神经元中PCD的这一基因表达成分进行了分析。我们发现数百个基因在离散波中受到显著调控，包括其蛋白质产物参与PCD的已知基因。所有模型均调控一组受限的基因，这表明诱导PCD的信号可调控大量基因组合（其中一个受限的子集可能在多种途径中共享）。
