Olvera-Carrillo Yadira, Van Bel Michiel, Van Hautegem Tom, Fendrych Matyáš, Huysmans Marlies, Simaskova Maria, van Durme Matthias, Buscaill Pierre, Rivas Susana, Coll Nuria S., Coppens Frederik, Maere Steven, Nowack Moritz K.
Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.).
Department of Plant Systems Biology, Vlaams Instituut voor Biotechnologie, and Department of Plant Biotechnology and Bioinformatics, Ghent University, 9052 Ghent, Belgium (Y.O.-C., M.V.B., T.V.H., M.F., M.H., M.S., M.v.D., F.C., S.M., M.K.N.);Institut National de la Recherche Agronomique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 441, and Centre National de la Recherche Scientifique, Laboratoire des Interactions Plantes-Microorganismes, Unité Mixte de Recherche 2594, F-31326 Castanet-Tolosan, France (P.B., S.R.); andCenter for Research in Agricultural Genomics, Bellaterra-Cerdanyola del Valles, 08193 Barcelona, Spain (N.S.C.)
Plant Physiol. 2015 Dec;169(4):2684-99. doi: 10.1104/pp.15.00769. Epub 2015 Oct 5.
A plethora of diverse programmed cell death (PCD) processes has been described in living organisms. In animals and plants, different forms of PCD play crucial roles in development, immunity, and responses to the environment. While the molecular control of some animal PCD forms such as apoptosis is known in great detail, we still know comparatively little about the regulation of the diverse types of plant PCD. In part, this deficiency in molecular understanding is caused by the lack of reliable reporters to detect PCD processes. Here, we addressed this issue by using a combination of bioinformatics approaches to identify commonly regulated genes during diverse plant PCD processes in Arabidopsis (Arabidopsis thaliana). Our results indicate that the transcriptional signatures of developmentally controlled cell death are largely distinct from the ones associated with environmentally induced cell death. Moreover, different cases of developmental PCD share a set of cell death-associated genes. Most of these genes are evolutionary conserved within the green plant lineage, arguing for an evolutionary conserved core machinery of developmental PCD. Based on this information, we established an array of specific promoter-reporter lines for developmental PCD in Arabidopsis. These PCD indicators represent a powerful resource that can be used in addition to established morphological and biochemical methods to detect and analyze PCD processes in vivo and in planta.
在生物体内，已经描述了大量多样的程序性细胞死亡（PCD）过程。在动物和植物中，不同形式的PCD在发育、免疫和对环境的反应中发挥着关键作用。虽然一些动物PCD形式（如凋亡）的分子控制已被详细了解，但我们对植物中多种类型PCD的调控仍知之甚少。部分原因是缺乏可靠的报告基因来检测PCD过程。在这里，我们通过结合生物信息学方法来识别拟南芥（Arabidopsis thaliana）不同PCD过程中共同调控的基因，从而解决了这个问题。我们的结果表明，发育控制的细胞死亡的转录特征与环境诱导的细胞死亡的转录特征在很大程度上是不同的。此外，不同的发育性PCD案例共享一组与细胞死亡相关的基因。这些基因中的大多数在绿色植物谱系中是进化保守的，这表明存在一个进化保守的发育性PCD核心机制。基于这些信息，我们建立了一系列用于拟南芥发育性PCD的特异性启动子 - 报告基因系。这些PCD指标是一种强大的资源，除了已有的形态学和生化方法外，还可用于在体内和植物中检测和分析PCD过程。
