人血清中糖原磷酸化酶的测定及其在心肌梗死诊断中的应用。

The assay of glycogen phosphorylase in human blood serum and its application to the diagnosis of myocardial infarction.

作者信息

Krause E G, Will H, Böhm M, Wollenberger A

出版信息

Clin Chim Acta. 1975 Jan 20;58(2):145-54. doi: 10.1016/s0009-8981(75)80006-4.

Abstract

Methods are described (a) for the estimation of glycogen phosphorylase activity (EC 2.4.1.1) in human blood serum based on the chemical determination of liberated orthophosphate or on the enzymic determination of glucose 1-phosphate in a coupled assay system and (b) for the electrophoretic separation of isophosphorylases I, II, and III in human. Glycogen phosphorylase activities ranging from 1.5 to 18 mU/ml were found in the serum of patients with acute myocardial infarction. In contrast, no glycogen phosphorylase activity was detected in the serum of healthy persons. The enzyme appears in the serum 4 hours after the onset of the infarction and reaches a maximum after 20 to 30 hours. Acrylamide gel electrophoresis of serum after a myocardial infarction revealed only muscle isophosphorylase I, the isoenzyme characteristic of the heart. No phosphorylase activity was detected in serum of patients with angina pectoris, endocarditis, and uncomplicative congestive heart failure. From these findings it appears that the new serum enzyme test may prove to be a valuable addition to presently existing methods for the early differential diagnosis of acute myocardial infarction.

摘要

描述了以下方法

(a)基于对释放的正磷酸盐的化学测定或在偶联测定系统中对1-磷酸葡萄糖的酶促测定来估算人血清中糖原磷酸化酶活性（EC 2.4.1.1），以及(b)对人异磷酸化酶I、II和III进行电泳分离。在急性心肌梗死患者的血清中发现糖原磷酸化酶活性范围为1.5至18 mU/ml。相比之下，在健康人的血清中未检测到糖原磷酸化酶活性。该酶在梗死发作后4小时出现在血清中，并在20至30小时后达到最大值。心肌梗死后血清的丙烯酰胺凝胶电泳仅显示肌肉异磷酸化酶I，即心脏特有的同工酶。在心绞痛、心内膜炎和无并发症的充血性心力衰竭患者的血清中未检测到磷酸化酶活性。从这些发现来看，新的血清酶检测可能被证明是对目前用于急性心肌梗死早期鉴别诊断的现有方法的有价值补充。