Plagemann P G
Department of Microbiology, University of Minnesota, Minneapolis, MN 55455, USA.
Virus Res. 2001 Apr;74(1-2):47-52. doi: 10.1016/s0168-1702(00)00247-1.
MRNA2 of the arteriviruses lactate dehydrogenase-elevating virus (LDV) and equine arteritis virus (EAV) encodes two proteins that are read in different frames, an about 26 kDa minor envelope glycoprotein and an about 8 kDa protein that lacks N-glycosylation sites and a signal peptide, but possesses a central hydrophobic segment. Recent studies have shown that both proteins of EAV are translated from mRNA 2 in EAV infected BHK cells, that the 8 kDa protein is membrane associated and that small amounts of it are recovered in purified virions (Snijder, E.J., van Tol, H., Pederson, K.W., Raamsman, M.J.B., de Vries, A.A.F., 1999. Identification of a novel structural protein of arteriviruses. J. Virol. 73, 6335-6345). The authors concluded that the 8 kDa protein is another arterivirus envelope protein and designated it E protein. However, we have not detected a significant level of an 8 kDa protein in LDV virions and thus conclude that it is not a structural virion component.
动脉炎病毒乳酸脱氢酶升高病毒（LDV）和马动脉炎病毒（EAV）的MRNA2编码两种在不同阅读框中读取的蛋白质，一种约26 kDa的小囊膜糖蛋白和一种约8 kDa的蛋白质，该蛋白质缺乏N-糖基化位点和信号肽，但具有一个中央疏水片段。最近的研究表明，EAV的这两种蛋白质均在感染EAV的BHK细胞中从mRNA 2翻译而来，8 kDa的蛋白质与膜相关，并且在纯化的病毒粒子中可回收少量该蛋白（斯奈德，E.J.，范托尔，H.，佩德森，K.W.，拉姆斯曼，M.J.B.，德弗里斯，A.A.F.，1999年。动脉炎病毒一种新型结构蛋白的鉴定。《病毒学杂志》73卷，6335 - 6345页）。作者得出结论，8 kDa的蛋白质是另一种动脉炎病毒囊膜蛋白，并将其命名为E蛋白。然而，我们在LDV病毒粒子中未检测到显著水平的8 kDa蛋白质，因此得出结论，它不是病毒粒子的结构成分。
