Wu Wai-Hong, Fang Ying, Rowland Raymond R R, Lawson Steven R, Christopher-Hennings Jane, Yoon Kyoung-Jin, Nelson Eric A
Department of Veterinary Science, South Dakota State University, Box 2175, Brookings, SD 57007, USA.
Virus Res. 2005 Dec;114(1-2):177-81. doi: 10.1016/j.virusres.2005.06.014. Epub 2005 Aug 10.
Porcine reproductive and respiratory syndrome virus (PRRSV) ORF2 contains an internal ORF that codes for a small non-glycosylated protein known as 2b. Previous work had identified the presence of a 10kDa 2b protein in virus-infected cells and the induction of an anti-2b response in PRRSV-infected pigs, as well as a possible association of 2b with the virion (, Virology 287:183-191). In this study, we utilized two experimental approaches, including the use of a 2b peptide-specific monoclonal antibody, to demonstrate that the PRRSV 2b protein is an integral component of the PRRSV virion. This study suggests that 2b in PRRSV is similar to the E protein in EAV and forms a minor structural component of the virion.
猪繁殖与呼吸综合征病毒（PRRSV）的开放阅读框2（ORF2）包含一个内部开放阅读框，其编码一种名为2b的非糖基化小蛋白。先前的研究已确定在病毒感染细胞中存在10 kDa的2b蛋白，并且在PRRSV感染的猪中诱导了抗2b反应，以及2b与病毒粒子可能存在关联（《病毒学》287:183 - 191）。在本研究中，我们采用了两种实验方法，包括使用2b肽特异性单克隆抗体，以证明PRRSV 2b蛋白是PRRSV病毒粒子的一个组成部分。这项研究表明，PRRSV中的2b与马动脉炎病毒（EAV）中的E蛋白相似，是病毒粒子的一个次要结构成分。
