Murota K, Kawada T, Matsui N, Sakakibara M, Takahashi N, Fushiki T
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Japan.
J Nutr Sci Vitaminol (Tokyo). 2000 Dec;46(6):302-8. doi: 10.3177/jnsv.46.302.
Long-chain fatty acids are important nutrients, but obesity is the most common nutritional disorder in humans. In this study we investigated the effect of oleyl alcohol on the intestinal long-chain fatty acid absorption in rats. We administered [14C]oleic acid and oleyl alcohol as lipid emulsion intraduodenally in unanesthetized lymph-cannulated rats and measured the lymphatic output of oleic acid. Second, we orally administered lipid emulsion with a stomach tube and measured the luminal and mucosal oleic acid residues. Furthermore, rats were fed oleyl alcohol as a dietary component for 20 days, and fecal lipid and the weight of adipose tissues were measured. In lymph-cannulated rats, triglyceride and [14C]oleic acid output in the lymph were significantly lower in the presence of oleyl alcohol when compared with the absence of oleyl alcohol in a dose-dependent manner. The radioactivity remaining in the intestinal lumen was more strongly detected in rats that had been orally administered oleyl alcohol than in the controls. The feces of rats fed an oleyl-alcohol-added diet contained much higher amounts of lipids, and the weights of their adipose tissues were significantly lower than in the control group. These results suggest that oleyl alcohol inhibits the rat gastrointestinal absorption of long-chain fatty acids in vivo.
长链脂肪酸是重要的营养物质，但肥胖是人类最常见的营养紊乱问题。在本研究中，我们调查了油醇对大鼠肠道长链脂肪酸吸收的影响。我们在未麻醉的、已插入淋巴管的大鼠十二指肠内给予[14C]油酸和油醇作为脂质乳剂，并测量油酸的淋巴输出量。其次，我们通过胃管口服给予脂质乳剂，并测量管腔和黏膜中的油酸残留量。此外，将油醇作为饮食成分喂给大鼠20天，并测量粪便脂质和脂肪组织的重量。在插入淋巴管的大鼠中，与不存在油醇的情况相比，存在油醇时淋巴中甘油三酯和[14C]油酸的输出量以剂量依赖的方式显著降低。在口服油醇的大鼠中，肠道管腔中残留的放射性比对照组中检测到的更强。喂食添加油醇饮食的大鼠粪便中脂质含量高得多，其脂肪组织重量明显低于对照组。这些结果表明，油醇在体内抑制大鼠胃肠道对长链脂肪酸的吸收。
