Prince G A, Denamur F, Deschamps M, Garçon N, Prieels J P, Slaoui M, Thiriart C, Porter D D
Virion Systems Inc., Rockville, MD 20850, USA.
Vaccine. 2001 Feb 28;19(15-16):2048-54. doi: 10.1016/s0264-410x(00)00417-5.
The mechanisms by which administration of a formalin-inactivated respiratory syncytial virus vaccine resulted in enhanced disease among children after they later became naturally infected with the virus remains largely undefined. After immunization and live virus challenge, the cotton rat demonstrated the histopathologic marker of the enhanced disease, polymorphonuclear leukocyte infiltration of lung alveolar spaces. We now report that immunization with formalin-inactivated vaccine formulated with the adjuvant, 3-deacylated monophosphoryl lipid A, dramatically reduces or eliminates the polymorphonuclear leukocyte infiltration within the alveoli of cotton rats post-challenge. We suggest, that this or similar adjuvants may be beneficial components of candidate non-replicating respiratory syncytial virus vaccines, whose development has been hampered by safety concerns.
福尔马林灭活呼吸道合胞病毒疫苗接种后，儿童随后自然感染该病毒时病情反而加重，其机制在很大程度上仍不明确。免疫接种和活病毒攻击后，棉鼠出现了病情加重的组织病理学标志物，即肺泡腔内多形核白细胞浸润。我们现在报告，用佐剂3 - 脱酰基单磷酰脂质A配制的福尔马林灭活疫苗进行免疫接种，可显著减少或消除攻击后棉鼠肺泡内的多形核白细胞浸润。我们认为，这种或类似的佐剂可能是候选非复制型呼吸道合胞病毒疫苗的有益成分，这类疫苗的研发因安全性问题而受阻。
