Okuda T, Baes A U, Nishijima W, Okada M
Department of Environmental Science, Faculty of Engineering, Hiroshima University 1-4-1 Kagamiyama, Higashi-Hiroshima, Hiroshima, 739-8527 Japan.
Water Res. 2001 Feb;35(2):405-10. doi: 10.1016/s0043-1354(00)00290-6.
It is known that M. oleifera contains a natural coagulant in the seeds. In our previous research, the method using salt water to extract the active coagulation component from M. oleifera seeds was developed and compared with the conventional method using water. In this research, the active coagulation component was purified from a NaCl solution crude extract of Moringa oleifera seeds. The active component was isolated and purified from the crude extract through a sequence of steps that included salting-out by dialysis, removal of lipids and carbohydrates by homogenization with acetone, and anion exchange. Specific coagulation activity of the active material increased up to 34 times more than the crude extract after the ion exchange. The active component was not the same as that of water extract. The molecular weight was about 3000 Da. The Lowry method and the phenol-sulfuric acid method indicated that the active component was neither protein nor polysaccharide. The optimum pH of the purified active component for coagulation of turbidity was pH 8 and above. Different from the conventional water extracts, the active component can be used for waters with low turbidity without increase in the dissolved organic carbon concentration.
已知辣木籽中含有一种天然凝血剂。在我们之前的研究中，开发了用盐水从辣木籽中提取活性凝血成分的方法，并与传统的用水提取方法进行了比较。在本研究中，从辣木籽的氯化钠溶液粗提物中纯化活性凝血成分。通过一系列步骤从粗提物中分离和纯化活性成分，这些步骤包括透析盐析、用丙酮匀浆去除脂质和碳水化合物以及阴离子交换。离子交换后，活性物质的比凝血活性比粗提物提高了34倍。活性成分与水提取物不同。分子量约为3000道尔顿。洛瑞法和苯酚-硫酸法表明活性成分既不是蛋白质也不是多糖。纯化的活性成分对浊度进行凝聚的最佳pH值为8及以上。与传统的水提取物不同，活性成分可用于低浊度水体，且不会增加溶解有机碳浓度。
