Sutton M R, MacGillivray R T, Brew K
Eur J Biochem. 1975 Feb 3;51(1):43-8. doi: 10.1111/j.1432-1033.1975.tb03904.x.
The amino acid sequences of three fragments obtained on cyanogen bromide cleavage of human transferrin have been determined. Two of the fragments are small (4 and 7 residues) and had not been isolated in previous studies of the CNBr fragments of transferrin. The sequence of the larger fragment (53 residues) was elucidated by examining peptides isolated from digests of the fragment with trypsin, chymotrypsin or thermolysin. This region of transferrin appears to contain the sites of three previously-reported substitutions in the D1 and D-chi genetic variants.
已确定了人转铁蛋白经溴化氰裂解得到的三个片段的氨基酸序列。其中两个片段较小（分别为4个和7个残基），在以往对转铁蛋白溴化氰片段的研究中未被分离出来。通过分析用胰蛋白酶、胰凝乳蛋白酶或嗜热菌蛋白酶消化该较大片段（53个残基）后分离得到的肽段，阐明了其序列。转铁蛋白的这一区域似乎包含了先前报道的D1和D-chi基因变体中三个取代位点。
