链球菌蛋白酶的一级结构。II. 溴化氰片段5的胰蛋白酶和糜蛋白酶肽段的分离、组成及氨基酸序列
Primary structure of streptococcal proteinase. II. Isolation, composition, and amino acid sequences of the tryptic and chymotryptic peptides of cyanogen bromide fragment 5.
作者信息
Tai J Y, Liu T Y
出版信息
J Biol Chem. 1976 Apr 10;251(7):1948-54.
PMID:1270416
Abstract
The amino acid sequence of the cyanogen bromide fragment 5 of streptococcal proteinase has been determined. This fragment comprises residues 130 to 253 of the proteinase chain. Six tryptic peptides were isolated from maleylated cyanogen bromide fragment 5, and their alignment was obtained by the overlap of chymotryptic peptides. Sequence analysis of tryptic, chymotryptic, and thermolysin peptides was performed by the 5-deimethylaminoaphthalene-1-sulfonyl technique and carboxypeptidases digestion.
摘要
已确定链球菌蛋白酶溴化氰片段5的氨基酸序列。该片段包含蛋白酶链的第130至253位残基。从马来酰化的溴化氰片段5中分离出六个胰蛋白酶肽段，并通过胰凝乳蛋白酶肽段的重叠获得它们的排列顺序。通过5-二甲基氨基萘-1-磺酰基技术和羧肽酶消化对胰蛋白酶、胰凝乳蛋白酶和嗜热菌蛋白酶肽段进行序列分析。
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