Sode K, Akaike E, Sugiura H, Tsugawa W
Department of Biotechnology, Tokyo University of Agriculture and Technology, 2-24-16 Nakamachi, Koganei, 184-8588, Tokyo, Japan.
FEBS Lett. 2001 Jan 26;489(1):42-5. doi: 10.1016/s0014-5793(00)02427-3.
We reported the preparation of a novel trehalose derivative based on enzymatic oxidation of trehalose by water-soluble glucose-3-dehydrogenase (G3DH) from marine bacterium Halomonas sp. alpha-15 cells. The product of G3DH enzymatic conversion was 3,3'-diketotrehalose (3,3'dkT), a novel trehalose derivative of which both third hydroxy groups of glucopyranosides were oxidized. 3,3'dkT was revealed to show an inhibitory effect toward pig-kidney and Bombyx mori trehalases. The IC(50) values of 3,3'dkT were 0.8 and 2.5 mM and K(i) values were 0.2 and 0.6 mM for pig-kidney and for B. mori trehalases, respectively. In addition, 3,3'dkT did not show any inhibitory effect on both maltase and mannosidase activities. Therefore, 3,3'dkT was a specific inhibitor of trehalases.
