Cottrell G A, Jeziorski M C, Green K A
School of Biology, University of St. Andrews, Fife, UK.
FEBS Lett. 2001 Jan 26;489(1):71-4. doi: 10.1016/s0014-5793(01)02081-6.
The second FMRFamide-gated Na(+) channel (HtFaNaC), from Helisoma trivolvis, has been cloned. HtFaNaC has some different pharmacological properties to HaFaNaC, from Helix aspersa, which has enabled a rational approach to be made to start to identify the FMRFamide recognition site. Several chimeras were made by switching sections between the channels. The differences in sensitivity to FMRFamide, and amiloride, were assessed after expression in Xenopus oocytes. The data suggest that a recognition site for FMRFamide, and the potentiating action of amiloride, resides in a sequence of about 120 amino acids in the extracellular loop proximal to the first transmembrane segment.
已克隆出来自三角帆蚌的第二个FMRF酰胺门控性钠离子通道（HtFaNaC）。HtFaNaC与来自褐云玛瑙螺的HaFaNaC具有一些不同的药理学特性，这使得我们能够采取合理的方法来开始确定FMRF酰胺识别位点。通过在通道之间切换片段构建了几种嵌合体。在非洲爪蟾卵母细胞中表达后，评估了对FMRF酰胺和阿米洛利的敏感性差异。数据表明，FMRF酰胺的识别位点以及阿米洛利的增强作用位于靠近第一个跨膜片段的细胞外环中约120个氨基酸的序列中。
