硫酸软骨素中寡糖的表征。还原二糖和四糖的（1）H-NMR和（13）C-NMR研究。

Characterization of oligosaccharides from the chondroitin sulfates. (1)H-NMR and (13)C-NMR studies of reduced disaccharides and tetrasaccharides.

作者信息

Huckerby T N, Lauder R M, Brown G M, Nieduszynski I A, Anderson K, Boocock J, Sandall P L, Weeks S D

机构信息

The Polymer Centre, School of Physics and Chemistry, Lancaster University, UK.

出版信息

Eur J Biochem. 2001 Mar;268(5):1181-9. doi: 10.1046/j.1432-1327.2001.01948.x.

DOI:10.1046/j.1432-1327.2001.01948.x

PMID:11231269

Abstract

Chondroitin sulfates were fragmented using the enzymes chondroitin sulfate ABC endolyase and chondroitin ACII lyase; both disaccharide and tetrasaccharide fragments were isolated after reduction to the corresponding 2-deoxy-2-N-acetylamino-D-galactitol (GalNAc-ol) form. These have the structures: Delta UA(beta 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc6S-ol, Delta UA2S(beta 1--3)GalNAc6S-ol, Delta UA(beta 1--3)GalNAc4S(beta 1--4)L-IdoA(alpha 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc4S(beta 1--4)GlcA(beta 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc4S-ol, Delta UA(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc6S-ol, Delta UA2S(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc4S-ol and Delta UA2S(beta 1--3)GalNAc6S(beta 1--4)GlcA(beta 1--3)GalNAc6S-ol, where Delta UA represents a 4,5-unsaturated hexuronic acid (4-deoxy-alpha-Lthreo-hex-4-enepyranosyluronic acid) and 6S/4S/2S represent O-ester sulfate groups at C6/C4/C2 sites. Complete (1)H-NMR and (13)C-NMR data are derived for these species, which may help to alleviate some of the significant difficulties resulting from signal complexity that are currently hindering the characterization and assignment of major and minor structural components within chondroitin sulfate and dermatan sulfate polymers.

摘要

使用硫酸软骨素ABC内切酶和硫酸软骨素ACII裂解酶对硫酸软骨素进行片段化处理；在还原为相应的2-脱氧-2-N-乙酰氨基-D-半乳糖醇（GalNAc-ol）形式后，分离出二糖和四糖片段。它们具有以下结构：ΔUA(β1→3)GalNAc4S-ol、ΔUA(β1→3)GalNAc6S-ol、ΔUA2S(β1→3)GalNAc6S-ol、ΔUA(β1→3)GalNAc4S(β1→4)L-IdoA(α1→3)GalNAc4S-ol、ΔUA(β1→3)GalNAc4S(β1→4)GlcA(β1→3)GalNAc4S-ol、ΔUA(β1→3)GalNAc6S(β1→4)GlcA(β1→3)GalNAc4S-ol、ΔUA(β1→3)GalNAc6S(β1→4)GlcA(β1→3)GalNAc6S-ol、ΔUA2S(β1→3)GalNAc6S(β1→4)GlcA(β1→3)GalNAc4S-ol和ΔUA2S(β1→3)GalNAc6S(β1→4)GlcA(β1→3)GalNAc6S-ol，其中ΔUA代表4,5-不饱和己糖醛酸（4-脱氧-α-L-苏式-己-4-烯吡喃糖醛酸），6S/4S/2S代表C6/C4/C2位点上的O-酯硫酸基团。已得出这些物质完整的(1)H-NMR和(13)C-NMR数据，这可能有助于缓解目前因信号复杂性而导致的一些重大困难，这些困难目前阻碍了硫酸软骨素和硫酸皮肤素聚合物中主要和次要结构成分的表征和归属。