Yoshida N, Yoshikawa T, Yamaguchi T, Naito Y, Tanigawa T, Murase H, Kondo M
First Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
Antioxid Redox Signal. 1999 Winter;1(4):555-62. doi: 10.1089/ars.1999.1.4-555.
This study was designed to investigate the effects of water-soluble vitamin E derivative, 2-(alpha-D-glucopyranosyl)methyl-2,5,7,8-tetramethylchroman-6-ol (TMG), on experimental colitis in rats. Colitis was induced in male Wistar rats weighing 200 grams using an enema of trinitrobenzene sulfonic acid (TNBS) dissolved in 50% ethanol; 1 ml of TMG dissolved in physiological saline (0.2 mg/ml, 2 mg/ml, 20 mg/ml) was injected intraperitoneally every day for 1 week after the enema. The damage score, wet weight of the colon, and increase in body weight were estimated 1 week after the enema of TNBS. Thiobarbituric acid-reactive substances (TBA-RS), an index of lipid peroxidation, and the level of alpha-tocopherol or TMG in the colonic mucosa were measured 1 week after the induction of colitis. As a result, increase in body weight was inhibited by the induction of colitis, although the inhibition was reduced in the group treated with TMG. The damage score, wet weight and TBA-RS were increased significantly in the colitis group; however, they were inhibited by the administration of TMG. The alpha-tocopherol level in the colonic mucosa was reduced by the induction of colitis, wheres TMG could not be detected in the colonic mucosa of rats treated with TMG. These results suggest that TMG is effective for the treatment of colitis in rats induced by TNBS.
本研究旨在探讨水溶性维生素E衍生物2-(α-D-吡喃葡萄糖基)甲基-2,5,7,8-四甲基色满-6-醇（TMG）对大鼠实验性结肠炎的影响。采用溶于50%乙醇的三硝基苯磺酸（TNBS）灌肠法，对体重200克的雄性Wistar大鼠诱导结肠炎；灌肠后1周，每天腹腔注射1毫升溶于生理盐水的TMG（0.2毫克/毫升、2毫克/毫升、20毫克/毫升），持续1周。TNBS灌肠1周后，评估损伤评分、结肠湿重和体重增加情况。在结肠炎诱导1周后，测量脂质过氧化指标硫代巴比妥酸反应性物质（TBA-RS）以及结肠黏膜中α-生育酚或TMG的水平。结果显示，结肠炎诱导抑制了体重增加，不过在TMG治疗组中这种抑制作用有所减轻。结肠炎组的损伤评分、湿重和TBA-RS显著增加；然而，TMG给药可抑制这些指标。结肠炎诱导使结肠黏膜中的α-生育酚水平降低，而在接受TMG治疗的大鼠结肠黏膜中未检测到TMG。这些结果表明，TMG对TNBS诱导的大鼠结肠炎治疗有效。
