Faculty of Veterinary Medicine, Islamic Azad University, Karaj Branch, Iran.
Hum Exp Toxicol. 2010 Apr;29(4):287-95. doi: 10.1177/0960327110361754. Epub 2010 Feb 9.
Regarding the role of free radicals in pathogenesis of inflammatory bowel disease (IBD), we were interested to investigate the effects of Teucrium persicum with approved antioxidant and anti-inflammatory properties in an experimental model of colitis. Immunologic colitis was induced by rectal administration of a mixture of 2,4,6-trinitrobenzene sulphonic acid (TNBS) and ethanol through rubber cannula into rats. Three different doses of Teucrium (100, 200, and 400 mg/kg) were gavaged in a duration of 10 days to rats. Endpoint markers of colitis included macroscopic and microscopic examination of colon tissue and measuring colonic cells concentrations of tumor necrosis factor-alpha (TNF-alpha), interlukin-1beta (IL-1beta), total antioxidant power as ferric reducing antioxidant power (FRAP), myeloperoxidase (MPO), and lipid peroxidation as thiobarbitoric acid-reactive substance (TBARS). Teucrium at all doses improved both macroscopic and histological damages of rats with colitis. Teucrium reduced colonic MPO activity and concentrations of cellular lipid peroxides, TNF-alpha, and IL-1beta, with a concomitant increase in FRAP value in rats with colitis. It is concluded that beneficial effects of Teucrium in experimental colitis is mediated through its antioxidant and anti-inflammatory potentials. Examination of this herbal medicine in patients with IBD as a supplement would further reveal the potential of Teucrium.
关于自由基在炎症性肠病（IBD）发病机制中的作用，我们有兴趣研究具有已证实抗氧化和抗炎特性的贯叶连翘（Teucrium persicum）在结肠炎实验模型中的作用。通过橡胶套管将 2,4,6-三硝基苯磺酸（TNBS）和乙醇的混合物直肠给药到大鼠中，诱导免疫性结肠炎。用贯叶连翘（100、200 和 400mg/kg）灌胃大鼠 10 天，共三个不同剂量。结肠炎的终点标志物包括结肠组织的宏观和微观检查，以及测量结肠细胞中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）、总抗氧化能力（FRAP）、髓过氧化物酶（MPO）和脂质过氧化产物（TBARS）的浓度。贯叶连翘在所有剂量下均改善了结肠炎大鼠的宏观和组织学损伤。贯叶连翘降低了结肠炎大鼠结肠 MPO 活性和细胞脂质过氧化物、TNF-α 和 IL-1β的浓度，同时 FRAP 值升高。结论是贯叶连翘在实验性结肠炎中的有益作用是通过其抗氧化和抗炎潜力介导的。在 IBD 患者中作为补充剂检查这种草药将进一步揭示贯叶连翘的潜力。
