Dai J G, Wei H, Xiao Y B
Department of Thoratic Surgery of Xinqiao Hospital, Chongqing 400037, China.
Yi Chuan Xue Bao. 2001;28(2):115-9.
The genetic variation of mtDNA among 7 common laboratory inbred strains of mice was analyzed by PCR-RFLP and PCR-SSCP techniques. D-loop, tRNA(Met + Glu + Ile) and ND3 fragments of mtDNA from the mices showed no variation in 46 endonuclease sites; Deeply analyzed by PCR-SSCP, the D-loop 5' fragment and 3' fragment of mtDNA from these mice also show no genetic variation. Because of maternal mode of inheritance of mtDNA, the results indicate that only one female lineage contributed to the formation of all these common inbred strains of mice.
采用PCR-RFLP和PCR-SSCP技术分析了7种常见实验室近交系小鼠线粒体DNA（mtDNA）的遗传变异。这些小鼠mtDNA的D环、tRNA（Met + Glu + Ile）和ND3片段在46个内切酶位点上未显示出变异；通过PCR-SSCP深入分析，这些小鼠mtDNA的D环5'片段和3'片段也未显示出遗传变异。由于mtDNA的母系遗传模式，结果表明所有这些常见近交系小鼠的形成仅由一个雌性谱系贡献。
