基于线粒体DNA的PCR-RFLP分析推断三种同域分布的红点鲑属鱼类的遗传分化
[Genetic differentiation of three sympatric charr species from genus Salvelinus inferred from PCR-RFLP analysis of mitochondrial DNA].
作者信息
Oleĭnik A G, Skurikhina L A, Brykov Vl A
机构信息
Institute of Marine Biology, Russian Academy of Sciences, Vladivostok, 690041 Russia.
出版信息
Genetika. 2003 Aug;39(8):1099-105.
We examined differentiation of three sympatric charr species Dolly Varden charr Salvelinus malma Walbaum, white-spotted charr S. leucomaenis Pallas, and Levanidov charr S. levanidovi Chereshnev, Scopetz, Gudkov. Using restriction fragment length polymorphism analysis (RFLP), three mitochondrial DNA fragments (ND1/ND2, ND5/ND6, and Cytb/D-loop) amplified in PCR were compared. The divergence of mtDNA nucleotide sequences between S. levanidovi and S. leucomaenis was about 9.7%, between S. levanidovi and S. malma, 8.7%, and between S. malma and S. laecomaenis, 7.5%. These results indicate genetic isolation of the species examined and support the earlier suggestion on closeness of Levanidov charr to the common ancestor of the genus Salvelinus.
我们研究了三种同域分布的红点鲑物种
多莉瓦登红点鲑（Salvelinus malma Walbaum）、白斑红点鲑（S. leucomaenis Pallas）和列瓦尼多夫红点鲑（S. levanidovi Chereshnev, Scopetz, Gudkov）的分化情况。利用限制性片段长度多态性分析（RFLP），对在聚合酶链反应（PCR）中扩增出的三个线粒体DNA片段（ND1/ND2、ND5/ND6和Cytb/D-loop）进行了比较。列瓦尼多夫红点鲑和白斑红点鲑之间的线粒体DNA核苷酸序列差异约为9.7%，列瓦尼多夫红点鲑和多莉瓦登红点鲑之间为8.7%，多莉瓦登红点鲑和白斑红点鲑之间为7.5%。这些结果表明所研究的物种存在遗传隔离，并支持了之前关于列瓦尼多夫红点鲑与红点鲑属共同祖先亲缘关系较近的观点。
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