Hu K, Sun Y, Chen D, Zhang Y
Institute of Biophysics Academia Sinica, Beijing, China.
J Photochem Photobiol B. 2000 Nov;58(2-3):163-9. doi: 10.1016/s1011-1344(00)00125-1.
The decay rate of the Bacteriorhodopsin (BR) photocycle intermediate M412 and proton, the proton pump efficiency (H+/M412), the ratios of M412 to other intermediates and the rotational correlation time (tauc) in purple membrane (PM) fragments treated by the zwitterionic detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate (CHAPS) with different concentrations were studied. The results show that: (1) The largest effect of CHAPS on M412 decay rate and proton decay rate of BR, tauc of PM and the ratios of M412 to other intermediates in BR photocycle is in the range of its critical micelle concentration (CMC). This indicates that changes of the ratios of M412 to other intermediates, tauc, M412 decay and proton decay occur and are due to the variation of the lipid environment. (2) The dependency of proton yield on CHAPS concentrations is basically consistent with that of M412s%. This indicates the relation between proton pumping function and M412. These studies show the importance of maintaining a native environment.
研究了用不同浓度的两性离子去污剂3-[(3-胆酰胺丙基)-二甲基铵]-1-丙烷磺酸盐（CHAPS）处理的紫膜（PM）片段中细菌视紫红质（BR）光循环中间体M412和质子的衰减率、质子泵效率（H⁺/M412）、M412与其他中间体的比率以及旋转相关时间（tauc）。结果表明：（1）CHAPS对BR的M412衰减率和质子衰减率、PM的tauc以及BR光循环中M412与其他中间体的比率的最大影响在其临界胶束浓度（CMC）范围内。这表明M412与其他中间体的比率、tauc、M412衰减和质子衰减发生了变化，并且是由于脂质环境的变化。（2）质子产率对CHAPS浓度的依赖性与M412s%的依赖性基本一致。这表明质子泵功能与M412之间的关系。这些研究表明维持天然环境的重要性。
