Dracheva S, Bose S, Hendler R W
Laboratory of Cell Biology, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD 20892, USA.
FEBS Lett. 1996 Mar 11;382(1-2):209-12. doi: 10.1016/0014-5793(96)00181-0.
In native purple membrane (PM), there are approximately 1 squalene, 2 glycolipid sulfate (GLS), and 6 phospholipid (PL) molecules per bacteriorhodopsin (BR) monomer. Brief (approximately 2 min) exposure to 0.1% Triton X-100 removes about 25%, 20%, and 6% of squalenes, GLS, and PL, respectively (this paper) while causing profound changes in the BR photocycle, including the loss of 'photocooperativity'. The BR photocycle in Triton-treated PM can be restored to near normal behavior by reconstitution with native PM lipids. Isolated squalenes are not effective whereas PL alone partially restores normal photocycle characteristics.
在天然紫膜（PM）中，每个细菌视紫红质（BR）单体大约有1个角鲨烯、2个糖脂硫酸盐（GLS）和6个磷脂（PL）分子。将其短暂（约2分钟）暴露于0.1%的 Triton X-100中，分别去除了约25%、20%和6%的角鲨烯、GLS和PL（本文），同时导致BR光循环发生深刻变化，包括“光协同性”的丧失。用天然PM脂质重构可使经Triton处理的PM中的BR光循环恢复到接近正常行为。分离出的角鲨烯无效，而单独的PL可部分恢复正常光循环特征。
