Prete S P, Giuliani A, Iona E, Fattorini L, Orefici G, Franzese O, Bonmassar E, Graziani G
Department of Neuroscience, University of Rome Tor Vergata, Italy.
J Chemother. 2001 Feb;13(1):52-8. doi: 10.1179/joc.2001.13.1.52.
Non-peptide antigens (e.g. glycolipids of microbial origin) presented by monocyte-associated CD1 molecules to T cells appear to play an important role in host immunity against tuberculosis and other pathogenic bacteria. Since vaccination with Bacillus Calmette-Guerin (BCG) has limited efficacy, the influence of viable BCG organisms on the induction of CD1b antigen by granulocyte macrophage-colony stimulating factor (GM-CSF) has been tested in adherent mononuclear cells obtained from peripheral blood of healthy donors. The results indicate that the vaccine reduces substantially CD1b induction by GM-CSF. On the other hand, BCG was found to promote a slight increase in the expression of this molecule on target cells not exposed to GM-CSF. Attempts to reverse the antagonistic effects of BCG on GM-CSF with high concentrations of GM-CSF, alone, or associated with IL-4, were unsuccessful. Moreover, mycobacteria suppression by 10 microg/ml of rifampin, did not affect BCG influence on CD1b induction. The present results suggest that mycobacterium-induced impairment of the CD1 system could play a role in the unsatisfactory results obtained with BCG vaccination.
单核细胞相关的CD1分子呈递给T细胞的非肽抗原（如微生物来源的糖脂）似乎在宿主抗结核和其他病原菌的免疫中发挥重要作用。由于卡介苗（BCG）接种的疗效有限，因此在从健康供体外周血获得的贴壁单核细胞中测试了活卡介苗生物对粒细胞巨噬细胞集落刺激因子（GM-CSF）诱导CD1b抗原的影响。结果表明，该疫苗可显著降低GM-CSF对CD1b的诱导作用。另一方面，发现卡介苗可使未暴露于GM-CSF的靶细胞上该分子的表达略有增加。单独使用高浓度GM-CSF或与IL-4联合使用来逆转卡介苗对GM-CSF的拮抗作用均未成功。此外，10微克/毫升利福平对分枝杆菌的抑制作用并不影响卡介苗对CD1b诱导的影响。目前的结果表明，分枝杆菌诱导的CD1系统损伤可能是卡介苗接种效果不理想的原因之一。
