Luk'ianov E V, Viedlokha A, Olsnes S, Kozlov Iu V
Mol Biol (Mosk). 2001 Jan-Feb;35(1):136-45.
A new mRNA coding for the heparin-binding EGF-like growth factor (HB-EGF) was found in Vero cells. The corresponding cDNA had C-156 in place of T, which resulted in a loss of the NheI site and a substitution of Leu-33 with Pro in the HB-EGF precursor. The known and new forms of the precursor were accordingly termed L and P. A possible conformational change in the corresponding propeptide region were assumed to affect processing of soluble secreted HB-EGF. The L and P mRNAs are differently expressed in various cell lines and have the identical 5'-untranslated sequences. Possibly, they are transcribed from one promoter and then alternatively spliced. Stimulation of resting Vero cells with tetraphorbol ester (TPA) substantially increased production of the L form, decreased production of the P form, and did not affect expression of the total HB-EGF mRNA. This was associated with an increase in binding of the diphtheria toxin, suggesting that the L HB-EGF precursor acts as its receptor.
在Vero细胞中发现了一种编码肝素结合表皮生长因子样生长因子（HB-EGF）的新信使核糖核酸（mRNA）。相应的互补脱氧核糖核酸（cDNA）中，C取代了T，位置在第156位，这导致NheI位点缺失，且HB-EGF前体中第33位的亮氨酸被脯氨酸取代。因此，前体的已知形式和新形式分别被命名为L型和P型。假定相应前肽区域可能发生的构象变化会影响可溶性分泌型HB-EGF的加工过程。L型和P型mRNA在各种细胞系中的表达不同，但具有相同的5'非翻译序列。它们可能由一个启动子转录，然后进行可变剪接。用十四酰佛波醇乙酯（TPA）刺激静止的Vero细胞，可使L型产物大幅增加，P型产物减少，且不影响总HB-EGF mRNA的表达。这与白喉毒素结合增加有关，表明L型HB-EGF前体可作为其受体。
