Abraham J A, Damm D, Bajardi A, Miller J, Klagsbrun M, Ezekowitz R A
Scios Nova Inc., Mountain View, CA 94043.
Biochem Biophys Res Commun. 1993 Jan 15;190(1):125-33. doi: 10.1006/bbrc.1993.1020.
Clones were obtained that encode the rat and mouse forms of heparin-binding EGF-like growth factor (HB-EGF), a potent mitogen for smooth muscle cells, fibroblasts and keratinocytes that is proposed to be derived from a transmembrane precursor. Within the HB-EGF precursor sequences predicted from these cDNAs, the region corresponding to the secreted ("mature") factor was found to represent one of the least well conserved areas when compared to human or monkey HB-EGF (73-76% sequence identity). Regions of high sequence conservation included the proposed juxtamembrane and transmembrane domains, as well as a proposed heparin-binding region within the "mature" factor. Northern blotting experiments using the HB-EGF clones as probes revealed HB-EGF transcript expression in multiple tissues, particularly lung, skeletal muscle, brain, and heart.
获得了编码大鼠和小鼠形式的肝素结合表皮生长因子（HB-EGF）的克隆，HB-EGF是一种对平滑肌细胞、成纤维细胞和角质形成细胞有强效作用的促有丝分裂原，据推测它来源于一种跨膜前体。在从这些cDNA预测的HB-EGF前体序列中，与人类或猴HB-EGF相比，对应于分泌型（“成熟”）因子的区域是保守性最差的区域之一（序列同一性为73-76%）。高序列保守区域包括推测的近膜区和跨膜区，以及“成熟”因子内一个推测的肝素结合区域。使用HB-EGF克隆作为探针进行的Northern印迹实验显示，HB-EGF转录本在多个组织中表达，特别是在肺、骨骼肌、脑和心脏中。
