Pankratova E V, Deev I E, Polianovskiĭ O L
Mol Biol (Mosk). 2001 Jan-Feb;35(1):34-41.
It has been shown that pro-mRNA of transcription factor Oct-1 undergoes the tissue-specific splicing. The Oct-1L subform is synthesized in mouse lymphoid myeloma cells NS/0 of the B series and is not found in other somatic and embryonal cells. Initiation sites of oct-1R mRNA transcription are at positions -159 and -307 from the AUG codon of the oct-1R exon. In both cases, no TATA box was found in the region preceding these sites (25-30 bp). A different form of oct-1 mRNA (oct-1U) was also found in NS/0 cells, which is probably synthesized in all cell lines. This subform differs from oct-1R, in particular, by the structure of the 5T-terminal exon, which is the result of alternative splicing.
已经表明转录因子Oct-1的前体mRNA经历组织特异性剪接。Oct-1L亚型在B系列小鼠淋巴骨髓瘤细胞NS/0中合成，而在其他体细胞和胚胎细胞中未发现。oct-1R mRNA转录的起始位点位于oct-1R外显子AUG密码子上游-159和-307位置。在这两种情况下，在这些位点之前的区域（25 - 30 bp）中均未发现TATA框。在NS/0细胞中还发现了一种不同形式的oct-1 mRNA（oct-1U），它可能在所有细胞系中合成。这种亚型与oct-1R不同，特别是在5' - 末端外显子的结构上，这是选择性剪接的结果。
