German A B, Nekliudov A D
Moscow State University of Forest, Mytishchi, Moscow Oblast, 141005 Russia.
Prikl Biokhim Mikrobiol. 2001 Jan-Feb;37(1):63-6.
Immobilization of aminoacylase from Streptoverticillium olivoreticuli by incorporation into acrylamide gel has been investigated. The data showed that the process should be carried out at constant pH. Thermal inactivation of the immobilized enzyme under the reaction conditions was studied. The kinetics of enzymatic stereospecific deacylation of N-acetyl-DL-phenylalanine was analyzed. The results of this study may be used in the synthesis of amino acid enantiomers.
研究了通过包埋于丙烯酰胺凝胶中来固定化橄榄网状链霉菌氨基酰化酶的方法。数据表明该过程应在恒定pH值下进行。研究了固定化酶在反应条件下的热失活情况。分析了N-乙酰-DL-苯丙氨酸的酶促立体特异性脱酰化动力学。本研究结果可用于氨基酸对映体的合成。
