Oreshina M G, Penzikova G A, Levitov M M, Bartoshevich Iu E
Antibiotiki. 1982 Oct;27(10):728-32.
The procedure for isolation of acylases from the fermentation broth filtrates of 3 actinomycetous cultures was developed with a yield of 72-97 per cent and 11-19- fold purification of the preparations. Comparative study of substrate specificity of acylase preparations showed that all of them possessed 2 types of the activity, i.e. penicillin acylase and aminoacylase cones. It was found that phenoxymethylpenicillin and acetylamino acids were the preferable substrates for revealing the hydrolytic activity of the acylases among beta-lactam antibiotics and acyl derivatives of amino acids, respectively.
从3种放线菌培养物的发酵液滤液中分离酰基转移酶的方法已被开发出来，制备物的产率为72% - 97%，纯化倍数为11 - 19倍。对酰基转移酶制备物的底物特异性进行的比较研究表明，它们都具有两种活性，即青霉素酰基转移酶和氨酰基转移酶活性。研究发现，在β-内酰胺抗生素中，苯氧甲基青霉素是揭示酰基转移酶水解活性的优选底物；在氨基酸的酰基衍生物中，乙酰氨基酸是揭示酰基转移酶水解活性的优选底物。
